Pyridine Nucleotide-Dependent Dehydrogenases
نویسندگان
چکیده
منابع مشابه
The Reduced Pyridine Nucleotide of Human Erythrocytes Dehydrogenases
Kiese (1) reported the presence of an enzyme in erythrocytes that would catalyze the reduction of methemoglobin by reduced triphosphopyridine nucleotide in the presence of methylene blue. This enzyme was purified further by Huennekens et al. (2) and reported to be active both with TPNH and, to a lesser extent, with DPNH. It was postulated that this enzyme was responsible for the physiological m...
متن کاملStereospecificity of hydrogen transfer by pyridine nucleotide-linked hydroxysteroid dehydrogenases.
The present studies are concerned with the determination of the steric course of the transfer of hydrogen catalyzed by hydroxysteroid dehydrogenases. The classic work of Vennesland et al. (l-3) has demonstrated that pyridine nucleotide-linked dehydrogenases catalyze a direct and stereospecific transfer of hydrogen between the substrate and the para position of the nicotinamide ring of the nucle...
متن کاملProtein fluorescence of nicotinamide nucleotide-dependent dehydrogenases.
1. The decrease in the protein fluorescence (F) of Neurospora crassa glutamate dehydrogenase is linearly related to the increase in the fraction of the coenzyme sites occupied by NADPH (alpha) at pH6.35. Under these conditions NADPH causes this enzyme to dissociate to monomers. 2. There is a non-linear relationship of F to alpha for NADH binding to give the alcohol dehydrogenase-NADH-isobutyram...
متن کاملThe purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.
1. The general applicability of affinity chromatography to the purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors is illustrated with several examples taken from crude systems. 2. Methods for overcoming the inevitable loss of selectivity experienced with these polymers are suggested. Effective use of the appropriate nucleotide, the second substrate and oth...
متن کاملPyridine nucleotide-linked dehydrogenases and isozymes of normal rat breast and growing and regressing breast cancers.
Four pyridine nucleotide-linked dehydrogenases, glucose-6phosphate dehydrogenase (G6PDH),3 isocitric dehydrogenase (ICDH), lactic dehydrogenase (LDH), and malic dehydrogenase (MDH), and their isozymes have been studied in the normal virgin rat breast, in the breast of tumor-bearing rats, in the breast cancers themselves, and in these tissues following ovariectomy. Enzyme activity expressed per ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)80996-5